Inhibitory effects of copper on bacterial and fungal growth

  • Dr. Mohsin Ali Khan Secretary E.E.T, Department of Microbiology, Era's Lucknow Medical College and Hospital, Lucknow, UP, India
  • Dr. Shadma Yaqoob Associate Professor, Department of Microbiology, Era's Lucknow Medical College and Hospital, Lucknow, UP, India
Keywords: Copper, Bactericidal, Fungicidal, Nosocomial infection

Abstract

Introduction: Researchers conducted a literature, technology and patent search that traced the history of understanding the “bacteriostatic and sanitizing properties of copper and copper alloy surfaces” which demonstrated that copper, in very small quantities, has the Copper alloy surfaces have intrinsic properties to destroy a wide range of microorganisms. Today copper, in the form of plumbing tube, copper or copper-alloy surfaces proved to be a significant step in decreasing the fungal and bacterial infections in hospitals.

Aims and objective: To know the bactericidal and fungicidal properties of copper for its implication in various areas in preventing nosocomial infection.

Material and Methods: Eight sterile petri dishes (four for Blood agar media and four for MacConkey agar media) and two sterile 30 ml screw capped bottles (for Sabouraud’s Dextrose agar media) were taken. Sterile copper discs were placed in four plates of Blood agar and MacConkey agar media and other four plates were without copper discs. In the same way copper piece was placed in one of the bottle with Sabourauds Dextrose agar media. Pure growths of E. coli, Klebsiella and candida were inoculated and incubated.

Results: The plates with bacterial and fungal growth were reported accordingly. The growth was significantly reduced in Plates and bottles with copper discs.

Conclusion: Copper alloy surfaces have intrinsic properties to destroy a wide range of microorganisms so copper’s use in water supplies and surfaces are recommended.

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References

Airey P , Verran J. Potential use of copper as a hygienic surface. problems associated with cumulative soiling and cleaning. J. Hosp. Infect. 2007;67(3):272-278. Doi: https://doi.org/10.1016/j.jhin.2007.09.002.

Block SS. Disinfection, Sterilisation and Preservation. 2001; 9:1857.

Dollwet HHA, Sorenson JRJ. Historic uses of copper compounds in medicine.Trace Elements in Medicine.1985; 2.2:80–87.

Weber DJ, Rutala WA. Use of metals as microbicides in preventing infections in healthcare. Philadelphia: Lippincott Williams & Wilkins .2001:415-30.

Sudha VB, Singh KO, Prasad SR, Venkatasubramanian P. Killing of enteric bacteria in drinking water by a copper device for use in the home: laboratory evidence. Trans R Soc Trop Med Hyg. 2009 Aug;103(8):819-22. doi: https://doi.org/10.1016/j.trstmh.2009.01.019. Epub 2009 Feb 23.

Dick RJ, Wray JA, Johnston HN. A Literature and Technology Search on the Bacteriostatic and Sanitizing Properties of Copper and Copper Alloy Surfaces. Phase 1 Final Report, INCRA Project 212 ,June 29, 1973; contracted to Battelle Columbus Laboratories, Columbus, Ohio.

Thurman RB, Gerba CP.The Molecular Mechanisms of Copper and Silver Ion Disinfection of Bacteria and Viruses.CRC Critical Reviews in Environmental Control.1989;18(4):295–315. Doi: https://doi.org/10.1080/10643388909388351.

Sterritt RM, Lester JN. Interactions of heavy metals with bacteria. Sci Total Environ. 1980 Jan;14(1):5-17.

Samuni A, Aronovitch J, Godinger D, Chevion M, Czapski G. On the cytotoxicity of vitamin C and metal ions. A site-specific Fenton mechanism. Eur J Biochem. 1983 Dec 1;137(1-2):119-24.

Samuni A, Chevion M, Czapski G. Roles of Copper and Superoxide Anion Radicals in the Radiation-Induced Inactivation of T7 Bacteriophage. Radiat. Res. JSTOR 1984; 99(3):562-572.

Manzl C, Enrich J, Ebner H, Dallinger R, Krumschnabel G. Copper-induced formation of reactive oxygen species causes cell death and disruption of calcium homeostasis in trout hepatocytes. Journal of Toxicology.2004; 196.(1–2): 57–64. Doi: https://doi.org/10.1016/j.tox.2003.11.001.

Gregor G, Christopher R, Marc S. Metallic Copper as an Antimicrobial SurfaceAppl. Environ. Microbiol. March 2011 vol. 77 ( 5):1541-1547. doi: https://dx.doi.org/10.1128%2FAEM.02766-10.

Centers for Disease Control and Prevention. Dept. of Health and Human Services. http://www.cdc.gov.

Casey AL, Adams D, Karpanen TJ, Lambert PA, Cookson BD, Nightingale P, Miruszenko L, Shillam R, Christian P, Elliott TS. Role of copper in reducing hospital environment contamination. J Hosp Infect. 2010 Jan;74(1):72-7. doi: https://doi.org/10.1016/j.jhin.2009.08.018. Epub 2009 Nov 20.

Marais F, Mehtar S, Chalkley L. Antimicrobial efficacy of copper touch surfaces in reducing environmental bioburden in a South African community healthcare facility. J Hosp Infect. 2010 Jan;74(1):80-2. doi: https://doi.org/10.1016/j.jhin.2009.07.010. Epub 2009 Sep 25.

Christophe E S, Christian G E, Davide Q, Dylan W D, Christopher J C, and Gregor G. Bacterial Killing by Dry Metallic Copper Surfaces. Appl. Environ. Microbiol. 2011; 77(3): 794-802.doi: https://doi.org/10.1128/AEM.01599-10.

Kuhn PJ. Doorknobs: a source of nosocomial infection. Diagnost Med 1983:62e63.

Michels HT, Wilks SA, Noyce JO, Keevil C.W. Copper Alloys for Human Infectious Disease Control. Presented at Materials Science and Technology Conference, September 2005; 25–28, Pittsburgh, PA. Copper for the 21st Century Symposium.

Wilks SA, Michels H, Keevil CW. The survival of Escherichia coli O157 on a range of metal surfaces. International Journal of Food Microbiology .2005;105(3):445–54. Doi: https://doi.org/10.1016/j.ijfoodmicro.2005.04.021.

Espírito Santo C, Taudte N, Nies DH, Grass G. Contribution of copper ion resistance to survival of Escherichia coli on metallic copper surfaces. Appl Environ Microbiol. 2008 Feb;74(4):977-86. Epub 2007 Dec 21.

Wells, F., Midwest Res Inst 2001, 348(348C/348D), 48.

Weaver L, Michels HT, Keevil CW. Potential for preventing spread of fungi in air-conditioning systems constructed using copper instead of aluminium.Lett Appl Microbiol. 2010 Jan;50(1):18-23. doi: https://doi.org/10.1111/j.1472-765X.2009.02753.x.

Inhibitory effects of copper on bacterial and fungal growth
CITATION
DOI: 10.17511/ijmrr.2017.i05.05
Published: 2017-05-31
How to Cite
1.
Ali Khan M, Yaqoob S. Inhibitory effects of copper on bacterial and fungal growth. Int J Med Res Rev [Internet]. 2017May31 [cited 2024Dec.22];5(5):466-71. Available from: https://ijmrr.medresearch.in/index.php/ijmrr/article/view/867
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