H-NMR based serum metabolomic signatures imperative in retinalneurodegeneration and development of Diabetic Retinopathy

  • Dr Arpan Guha Mazumder School of Medical Science and Technology, Indian nstitute of Technology, Kharagpur, India
  • Dr Sambuddha Ghosh Associate Professor, Ophthalmology, Calcutta National Medical College, Gorachand Road, Kolkata, India
  • Dr Swarnendu Bag School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India
  • Dr Sumanta Bera Fellowship Trainee, College Road, Nungambakkam, Chennai, Tamil Nadu, India
  • Dr Srutarshi Ghosh Internee, Calcutta Medical College, College Street, Kolkata, India
  • Dr Anirban Mukherjee Department of Electrical Engineering, Indian Institute of Technology, Kharagpur, India
  • Dr Jyotirmoy Chatterjee School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, India
DOI: https://doi.org/10.17511/ijmrr.2016.i06.19
Keywords: Serum metabolomics, H-NMR Spectroscopy, Abnormal polyol metabolism, Retinal neurodegeneration, Diabetic Retinopathy, Retinol metabolism

Abstract

Aim: To identify serum metabolomic fingerprints using 1H NMR in Diabetic Retinopathy (DR).

Methods and Materials: 1H-NMR was performed on 32 subjects [11 type 2 diabetic patients each without DR (group A) and with DR (group B) along with 10 control (group C)].

Results:The study unraveled 1H-NMR based serum metabolomic fingerprints of diabetic retinopathy showed significant variations in Ribitol, D-glucose, Fructose-6-phosphate, Uridine Diphosphate-N-acetyl glucosamine and Glycerophosphocholine. The study envisaged that abnormal polyol metabolism and accumulation of ribitol contributory to development of diabetic retinopathy.Presence of Uridine Diphosphate-N-acetylglucosamine in blood has been also confirmed its probable role in progressive neurodegeneration in diabetic retinopathy. NMR spectra also indicated that downregulation of glycerophosphocholine is directly related to retinol metabolism and subsequent unusual chromophore synthesis facilitating retinal degeneration.

Conclusion:Five metabolomic fingerprints have been identified and found to be unique in Diabetic Retinopathy (DR).

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H-NMR based serum metabolomic signatures imperative in retinalneurodegeneration and development of Diabetic Retinopathy
CITATION
DOI: 10.17511/ijmrr.2016.i06.19
Published: 2016-06-30
How to Cite
1.
Guha Mazumder A, Ghosh S, Bag S, Bera S, Ghosh S, Mukherjee A, Chatterjee J. H-NMR based serum metabolomic signatures imperative in retinalneurodegeneration and development of Diabetic Retinopathy. Int J Med Res Rev [Internet]. 2016Jun.30 [cited 2024Nov.8];4(6):976-81. Available from: https://ijmrr.medresearch.in/index.php/ijmrr/article/view/590
Issue
Vol 4 No 6 (2016): JUNE
Section
Original Article