Bone marrow aspiration in hematology and oncology-10cc versus 50cc syringe
Abstract
Aims: To compare efficacy of Bone Marrow Aspiration (BMA) using 10cc syringe versus 50cc syringe in hematology and oncology.
Introduction: Bone Marrow Aspiration is an important investigation in diagnostic hematology and oncology. While the procedure of BMA has been standardized, the optimal size of the syringe to be used is still uncertain.
Methods: It is a retrospective analysis involving 300 patients undergoing diagnostic Bone Marrow Aspiration (BMA) at our institute over a period of two years.10 cc syringe was used in 149 patients and 50cc syringe in 151 patients. The results were analyzed using MEDCALC version 7.5 for WINDOWS software.
Results: 65 patients (43%) in the 50cc arm and 60 patients (40%) in 10cc arm had adequate volume of aspirate which resulted in a conclusive report suggesting that the 50cc syringe may be better than 10cc syringe.The use of 50cc syringe also resulted in lesser number of inadequate aspirate, inconclusive reports and dry taps. The differences between the 10cc and 50cc arms were not statistically significant though.
Conclusion: Bone Marrow Aspiration (BMA) using 10 cc syringe was as effective as BMA using 50cc syringe in diagnosis of hematology and oncology.
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References
2. Lee SH, Erber WN, Porwit A, Tomonaga M, Peterson LC; International Council for Standardization In Hematology. ICSH guidelines for the standardization of bone marrow specimens and reports.Int J Lab Hematol. 2008 Oct;30(5):349-64. doi: 10.1111/j.1751-553X.2008.01100.x. [PubMed]
3. Hernigou P, Homma Y, FlouzatLachaniette CH, Poignard A, Allain J, Chevallier N, Rouard H. Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells.IntOrthop. 2013 Nov;37(11):2279-87. doi: 10.1007/s00264-013-2017-z. Epub 2013 Jul 24. [PubMed]
4. Tripathy S, Dudani S. Comparative Evaluation of Simultaneous Bone Marrow Aspiration and Trephine Biopsy - Experience From Routine Hematology Practice, Indian Journal of Clinical Practice, 2013, Vol. 24, No. 5. [PubMed]
5. Muschler GF, Boehm C, Easley K. Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume. J Bone Joint Surg Am. 1997 Nov;79(11):1699-709.
6. Owen M, Friedenstein AJ.Stromalstem cells: marrow-derivedosteogenic precursors. Ciba Found Symp.1988;136:42-60. [PubMed]
7. Riley RS, Hogan TF, Pavot DR, Forysthe R, Massey D, Smith E, Wright L Jr, Ben-Ezra JM. A pathologist's perspective on bone marrow aspiration and biopsy: I. Performing a bone marrow examination. J Clin Lab Anal. 2004;18(2):70-90. [PubMed]
8. Humphries JE. Drytapbone marrowaspiration: clinical significance. Am JHematol. 1990 Dec;35(4):247-50. [PubMed]
9. Ozkalemkas F, Ali R, Ozkocaman V, Ozcelik T, Ozan U, Ozturk H, Kurt E, Evrensel T, Yerci O, Tunali A. The bone marrow aspirate and biopsy in the diagnosis of unsuspected nonhematologic malignancy: a clinical study of 19 cases. BMC Cancer. 2005 Nov 1;5:144. [PubMed]
10. Charles KS, Winfield DA, Angel C, Goepel J. Audit of bone marrow aspirates and trephine biopsies in multiple myeloma--a single centre study. Clin Lab Haematol. 2004 Dec;26(6):403-6.
11. Roeckel IE. Diagnosis of metastatic carcinoma by bone marrow biopsy versus bone marrow aspiration.Ann Clin Lab Sci. 1974 May-Jun;4(3):193-7. [PubMed]
12. Marec-Berard P, Montella A, Schmitt C, et al. Evaluation oflidocaine infiltration efficiency for pain relief during bone marrow aspiration in children with cancer. Adv Pediatr Res,2015,2:7.doi:10.12715/apr.2015.2.713. [PubMed]
13. Basu D, DasS, JacobSE, KarR, MishraP, hematopoietic malignancies metastasing to the bone marrow: A 5 year record-based descriptive study from a tertiary care Centre in South India,Indian Journal of Cancer, January-March, 2014, Vol. 51, No. 1, pp. 30-34,DOI: 10.4103/0019-509X.134614.
14. Brahmbhatt B, Parikh B, Shah M. Bone Marrow involvement by Metastatic solid Tumors Gujarat Medical Journal, 2014, August Vol.69 No. 2; 54-57.
15. Singh MK, Goel MM, Singh U S, Dalela D, Shankhwar S N, Kumar A. Detection of bone marrow metastases in prostate cancer: Role of trephine biopsy and Immunohistochemistry. Clin Cancer Investig J 2013; 2:319-24.
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