Effect of parachlorophenylalanine, a specific 5-HT depleter on fluoxetine and d-fenfluramine induced penile erections in rats

  • Dr More Kanhaiah Assistant Professor, Dept of Pharmacology, Travancore Medical College, Medicity, Kollam, Kerala, India
  • Dr Vandana M Thorat Professor, Dept of Pharmacology, Krishna Institute of Medical Sciences University Karad, Maharashtra, India
  • Dr Anjali R Shinde Assistant Professor, Dept of Pharmacology, PIMS, Islampur, Maharashtra, India
  • Dr Santosh G Gursale Professor, Dept of Pharmacology, IMSR Medical College, Mayani, Maharashtra, India
DOI: https://doi.org/10.17511/ijmrr.2015.i9.178
Keywords: Fluoxetine (FLU), d-Fenfluramine (d-FEN), Parachlorophenylalanine (PCPA), Penile Erections (PEs), Penile Erections (PEs)Intraperitoneal injection (ip), Metachlorophenylpiperazine (mCPP), 5-Hydroxytriptamine (5-HT)

Abstract

Objective: To determine whether fluoxetine (FLU) induces penile erection (PE) in rats either indirectly via its 5-HT neuronal reuptake inhibiting activity or directly by stimulating the 5-HT2c receptors in the brain.

Method: The effect of pre-treatment with para-Chlorophenylalanine (PCPA) on fluoxetine and d-fenfluramine (d-FEN) induced PEs (Penile erections) was studied in albino rats.

Results: FLU (10mg/kg) and d-FEN (2.5mg/kg) treated groups exhibited a significant increase in number of PEs as compared to their respective control (DW treated) groups. Pre-treatment with PCPA (100mg/kg/day) had no significant effect on the PEs induced by FLU (10mg/kg) but did significantly decrease the PEs induced by d-FEN (2.5 mg/kg).

Conclusion: Our results indicate that pre-treatment with selective 5-HT depleter PCPA (100mg/kg/day) significantly decreased PEs induced by d-FEN (2.5mg/kg) but had no significant effect on the number of PEs induced by FLU (10 mg/kg). This indicates that the PE inducing effect of FLU is probably due to the stimulation of 5HT2C receptors.

Downloads

Download data is not yet available.

References

1. Berendsen HH, Jenck F, Broekkamp CL.Involvement of 5-HT1C-receptors in drug-induced penile erections in rats. Psychopharmacology (Berl). 1990;101(1):57-61.2.
MartinGR, HumphreyPPReceptors for 5hydroxytrypta mine: current perspectives on classification and nomenc lature. Neuropharmacology. 1994 Mar-Apr;33(3-4):261-73.

3. Millan MJ, Peglion JL, Lavielle G, Perrin- Monneyron S 5-HT2C receptors mediate penile erections in rats: actions of novel and selective agonists and antagonists. Eur J Pharmacol. 1997 Apr23;325(1):9-12.

4. Kimura Y, Naitou Y, Wanibuchi F, Yamaguchi T. 5- HT(2C) receptor activation is a common mechanism on proerectile effects of apomorphine, oxytocin and
melanotan-II in rats. Eur J Pharmacol. 2008 Jul 28;589(1-3):157-62. doi: 10.1016/j.ejphar.2008.05.022. Epub 2008 May 24.

5. Berendsen HH, Broekkamp CL. Drug-induced penile erections in rats: indications of serotonin1B receptor mediation. Eur J Pharmacol. 1987 Mar 31;135(3):279-87.

6. Jenck F, Moreau JL, Mutel V, Martin JR, Haefely WE. Evidence for a role of 5-HT1C receptors in the antiserotonergic properties of some antidepressant drugs. Eur J Pharmacol. 1993 Feb 9;231(2):223-9.

7. Cooper JR, Bloom FE, Roth RH. The Biochemical basis of Neuropharmacology. 8th ed. New York: Oxford University Press; 2003. p. 271-320.

8. Wanibuchi F, Usuda S. Synergistic effects between D-1 and D-2 dopamine antagonists on catalepsy in rats. Psychopharmacology (Berl). 1990;102(3):339-42.

9. Trulson ME, Jacobs BL. Behavioral evidence for the rapid release of CNS serotonin by PCA and fenfluramine. Eur J Pharmacol. 1976 Mar;36(1):149-54.

10. Thorat VM, Gaonkar RK, Bhosale KB, Balsara JJ. Effects of dexfenfluramine on dopamine dependent behaviours in rats. Indian J Physiol Pharmacol. 2005 Jan;49(1): 39-48.

11. Page ME1, Detke MJ, Dalvi A, Kirby LG, Lucki I.Serotonergic mediation of the effects of fluoxetine, but not desipramine, in the rat forced swimming test.
Psychopharmacology (Berl). 1999 Nov;147(2):162-7.

12. Koe BK, Weissman A. p-Chlorophenylalanine: a specific depletor of brain serotonin. J Pharmacol Exp Ther. 1966 Dec;154(3):499-516.

13. Zhang X, Peng L, Chen Y, Hertz L. Stimulation of glycogenolysis in astrocytes by fluoxetine, an antidepressant acting like 5-HT. Neuroreport. 1993 Sep 10;4(11):1235-8.

14. Chen Y, Peng L, Zhang X, Stolzenburg JU, Hertz L.Further evidence that fluoxetine interacts with a 5-HT2C receptor in glial cells. Brain Res Bull. 1995;38(2):153-9.
CITATION
DOI: 10.17511/ijmrr.2015.i9.178
Published: 2015-10-31
How to Cite
1.
Kanhaiah M, M Thorat V, R Shinde A, G Gursale S. Effect of parachlorophenylalanine, a specific 5-HT depleter on fluoxetine and d-fenfluramine induced penile erections in rats. Int J Med Res Rev [Internet]. 2015Oct.31 [cited 2024Dec.23];3(9):959-63. Available from: https://ijmrr.medresearch.in/index.php/ijmrr/article/view/351
Issue
Vol 3 No 9 (2015): OCTOBER
Section
Original Article