Phenotypic detection of ESBL and MBL in Gram Negative bacilli isolated from clinical specimens
Keywords:
Extended spectrum –beta lactamases, Metallobetalactamases, Gram Negative Bacilli
Abstract
Introduction: Antimicrobial resistance is a growing threat worldwide. The prevalence of Extended spectrum beta lactamases (ESBLs) and Metallobetalactamases (MBL) among Gram negative bacilli constitutes a serious threat to current beta-lactam therapy leading to treatment failure.
Material and Methods: ESBL was detected by double disc diffusion test using ceftazidime alone and in combination with clavulanic acid. MBL detection was done by Imipenem EDTA combined disc diffusion test.
Results: Out of 549 Gram negative bacilli resistant to 3G cephalosporin, 179(32.60%) were ESBL producers and out of 236 Gram negative bacilli resistant to carbapenem, 47(19.19%) were MBL producers. ESBL production was observed in E.coli, Klebsiella spp. Proteus spp and Citrobacter spp, while MBL production was observed in Pseudomonas aeruginosa, Acinetobacter spp, E.coli and Klebsiella spp isolated from various clinical samples.
Conclusion: Simple disc method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains.
Downloads
Download data is not yet available.
References
1. Coque TM, Baquero F, Canton R. Increasing prevalence of ESBL-producing Enterobacteriaceae in Europe. Euro Surveill. 2008 Nov 20;13(47). pii: 19044. [PubMed]
2. Peshattiwar P D, Peerapur.B V. ESBLand MBL Mediated Resistance in Pseudomonas aeruginosa. J. Clini. Diagnostic Res.2011; 5(8): 1552-1554. [PubMed]
3. Sridhar Rao PN, Basavarajappa KG, Krishna GL. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. Indian J Pathol Microbiol. 2008 Oct-Dec;51(4):497-9. [PubMed]
4. Chandel DS, Johnson JA, Chaudhry R, Sharma N, Shinkre N, Parida S, Misra PR, Panigrahi P. Extended-spectrum beta-lactamase-producing Gram-negative bacteria causing neonatal sepsis in India in rural and urban settings. J Med Microbiol. 2011 Apr;60(Pt 4):500-7. doi: 10.1099/jmm.0.027375-0. Epub 2010 Dec 23.
5. Rupp ME, Fey PD. Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs. 2003;63(4):353-65.
6. Rajput A, Prajapati B, Chauhan B, Shah A, Trivedi T and Kadam M. Prevalence of Metallo-beta-lactamases (MBL) producing Pseudomonas aeruginosa in a tertiary care Hospital. Indian. J. Basic.Appl. Med. Res. 2012; 1(4): 304-308.
7. Collee JG, Miles RS ,Watt B. Tests for identification of bacteria. In: Collee JG, Fraser AG, Marmion BP and Simmons A editors. Mackie and McCartney Practical Medical Microbiolgy. 14th ed. Haryana: Churchill- Livingstone;2006. p.131-149. [PubMed]
8. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-beta-lactamases: the quiet before the storm? Clin Microbiol Rev. 2005 Apr;18(2):306-25. [PubMed]
9. Bradford PA. Extended-Spectrum -Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin Microbiol Rev. 2001 Oct; 14(4): 933–951. doi: 10.1128/CMR.14.4.933-951.2001.
10. Agrawal P, Ghosh AN, Kumar S, Basu B, Kapila K. Prevalence of extended-spectrum beta-lactamases among Escherichia coli and Klebsiella pneumoniae isolates in a tertiary care hospital. Indian J Pathol Microbiol. 2008 Jan-Mar;51(1):139-42. [PubMed]
11. Tsering DC, Das S, Adhiakari L, Pal R, Singh TS. Extended Spectrum Beta-lactamase Detection in Gram-negative Bacilli of Nosocomial Origin. J Glob Infect Dis. 2009 Jul;1(2):87-92. doi: 10.4103/0974-777X.56247. [PubMed]
12. Shiju MP, Yashavanth R, Narendra N. Detection of Extended Spectrum Beta-Lactamase Production and Multidrug Resistance in Clinical Isolates of E. coli and K. pneumoniae in Mangalore. J Clin Diagnos Res 2010;4:2442-2445.
13. Rao SP, Rama PS, Gurushanthappa V, Manipura R, Srinivasan K Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae: A Multi-Centric Study Across Karnataka. J Lab Physicians. 2014 Jan;6(1):7-13. doi: 10.4103/0974-2727.129083.
14. Shashwati N, Kiran T, Dhanvijay AG. Study of extended spectrum β-lactamase producing Enterobacteriaceae and antibiotic coresistance in a tertiary care teaching hospital. J Nat Sci Biol Med. 2014 Jan;5(1):30-5. doi: 10.4103/0976-9668.127280. [PubMed]
15. Kusum M, Wongwanich S, Dhiraputra C, Pongpech P, Naenna P. Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a University Hospital, Thailand. J Med Assoc Thai. 2004 Sep;87(9):1029-33. [PubMed]
16. Kumar MS, Lakshmi V, Rajagopalan R. Occurrence of extended spectrum beta-lactamases among Enterobacteriaceae spp. isolated at a tertiary care institute. Indian J Med Microbiol. 2006 Jul;24(3):208-11. [PubMed]
17. Kumar SH, Baveja SM, Gore MA. Prevalence and risk factors of Metallo β-lactamase producing Pseudomonas aeruginosa and Acinetobacter species in burns and surgical wards in a tertiary care hospital. J Lab Physicians. 2012 Jan;4(1):39-42. doi: 10.4103/0974-2727.98670.
18. Rao SD, Kumar EA. Antimicrobial resistance and metallo β lactamase in gram-negative isolates of hospital-acquired burn wound infections. J Dr NTR Univ Health Sci 2013;2:181-5.
19. Dalela G Prevalence of Extended Spectrum Beta-Lactamase(ESBL) Producers among Gram Negative Bacilli from Various Clinical Isolates in a Tertiary Care Hospital at Jhalawar, Rajasthan, Indian. J. Clini.Diagnostic Res. 2012; 6(2): 182-187.
20. Behera B, Mathur P, Das A, Kapil A, Sharma V. An evaluation of four different phenotypic techniques for detection of metallo-beta-lactamase producing Pseudomonas aeruginosa. Indian J Med Microbiol. 2008 Jul-Sep;26(3):233-7.
2. Peshattiwar P D, Peerapur.B V. ESBLand MBL Mediated Resistance in Pseudomonas aeruginosa. J. Clini. Diagnostic Res.2011; 5(8): 1552-1554. [PubMed]
3. Sridhar Rao PN, Basavarajappa KG, Krishna GL. Detection of extended spectrum beta-lactamase from clinical isolates in Davangere. Indian J Pathol Microbiol. 2008 Oct-Dec;51(4):497-9. [PubMed]
4. Chandel DS, Johnson JA, Chaudhry R, Sharma N, Shinkre N, Parida S, Misra PR, Panigrahi P. Extended-spectrum beta-lactamase-producing Gram-negative bacteria causing neonatal sepsis in India in rural and urban settings. J Med Microbiol. 2011 Apr;60(Pt 4):500-7. doi: 10.1099/jmm.0.027375-0. Epub 2010 Dec 23.
5. Rupp ME, Fey PD. Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs. 2003;63(4):353-65.
6. Rajput A, Prajapati B, Chauhan B, Shah A, Trivedi T and Kadam M. Prevalence of Metallo-beta-lactamases (MBL) producing Pseudomonas aeruginosa in a tertiary care Hospital. Indian. J. Basic.Appl. Med. Res. 2012; 1(4): 304-308.
7. Collee JG, Miles RS ,Watt B. Tests for identification of bacteria. In: Collee JG, Fraser AG, Marmion BP and Simmons A editors. Mackie and McCartney Practical Medical Microbiolgy. 14th ed. Haryana: Churchill- Livingstone;2006. p.131-149. [PubMed]
8. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-beta-lactamases: the quiet before the storm? Clin Microbiol Rev. 2005 Apr;18(2):306-25. [PubMed]
9. Bradford PA. Extended-Spectrum -Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin Microbiol Rev. 2001 Oct; 14(4): 933–951. doi: 10.1128/CMR.14.4.933-951.2001.
10. Agrawal P, Ghosh AN, Kumar S, Basu B, Kapila K. Prevalence of extended-spectrum beta-lactamases among Escherichia coli and Klebsiella pneumoniae isolates in a tertiary care hospital. Indian J Pathol Microbiol. 2008 Jan-Mar;51(1):139-42. [PubMed]
11. Tsering DC, Das S, Adhiakari L, Pal R, Singh TS. Extended Spectrum Beta-lactamase Detection in Gram-negative Bacilli of Nosocomial Origin. J Glob Infect Dis. 2009 Jul;1(2):87-92. doi: 10.4103/0974-777X.56247. [PubMed]
12. Shiju MP, Yashavanth R, Narendra N. Detection of Extended Spectrum Beta-Lactamase Production and Multidrug Resistance in Clinical Isolates of E. coli and K. pneumoniae in Mangalore. J Clin Diagnos Res 2010;4:2442-2445.
13. Rao SP, Rama PS, Gurushanthappa V, Manipura R, Srinivasan K Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae: A Multi-Centric Study Across Karnataka. J Lab Physicians. 2014 Jan;6(1):7-13. doi: 10.4103/0974-2727.129083.
14. Shashwati N, Kiran T, Dhanvijay AG. Study of extended spectrum β-lactamase producing Enterobacteriaceae and antibiotic coresistance in a tertiary care teaching hospital. J Nat Sci Biol Med. 2014 Jan;5(1):30-5. doi: 10.4103/0976-9668.127280. [PubMed]
15. Kusum M, Wongwanich S, Dhiraputra C, Pongpech P, Naenna P. Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a University Hospital, Thailand. J Med Assoc Thai. 2004 Sep;87(9):1029-33. [PubMed]
16. Kumar MS, Lakshmi V, Rajagopalan R. Occurrence of extended spectrum beta-lactamases among Enterobacteriaceae spp. isolated at a tertiary care institute. Indian J Med Microbiol. 2006 Jul;24(3):208-11. [PubMed]
17. Kumar SH, Baveja SM, Gore MA. Prevalence and risk factors of Metallo β-lactamase producing Pseudomonas aeruginosa and Acinetobacter species in burns and surgical wards in a tertiary care hospital. J Lab Physicians. 2012 Jan;4(1):39-42. doi: 10.4103/0974-2727.98670.
18. Rao SD, Kumar EA. Antimicrobial resistance and metallo β lactamase in gram-negative isolates of hospital-acquired burn wound infections. J Dr NTR Univ Health Sci 2013;2:181-5.
19. Dalela G Prevalence of Extended Spectrum Beta-Lactamase(ESBL) Producers among Gram Negative Bacilli from Various Clinical Isolates in a Tertiary Care Hospital at Jhalawar, Rajasthan, Indian. J. Clini.Diagnostic Res. 2012; 6(2): 182-187.
20. Behera B, Mathur P, Das A, Kapil A, Sharma V. An evaluation of four different phenotypic techniques for detection of metallo-beta-lactamase producing Pseudomonas aeruginosa. Indian J Med Microbiol. 2008 Jul-Sep;26(3):233-7.
CITATION
DOI: 10.17511/ijmrr.2015.i8.163
Published: 2015-09-30
How to Cite
1.
Shivajirao Kamble D. Phenotypic detection of ESBL and MBL in Gram Negative bacilli isolated from clinical specimens. Int J Med Res Rev [Internet]. 2015Sep.30 [cited 2024Nov.23];3(8):866-70. Available from: https://ijmrr.medresearch.in/index.php/ijmrr/article/view/337
Section
Original Article
OAI - Open Archives Initiative
