Dengue Serotypes In Manipur – Findings From A Retrospective Analytical Study In A Tertiary Care Hospital

  • Laitonjam Anand Singh Virus Research & Diagnostic Laboratory, RIMS, Imphal
  • Thingujam Suson Virus Research & Diagnostic Laboratory, RIMS, Imphal
  • Chongtham Geeta Chanu Virus Research & Diagnostic Laboratory, RIMS, Imphal
  • Khuraijam Ranjana Devi Department of Microbiology, RIMS, Imphal
  • Heigrujam Rebachandra Singh Department of Microbiology, RIMS, Imphal
Keywords: Dengue virus, Dengue IgM ELISA, Dengue serotype (RT PCR)

Abstract

Objectives: As a part of on-going research programme on vector-borne viral diseases especially on Dengue, a retrospective analytical study on the occurrence and distribution of Dengue virus serotype(s) in the state of Manipur – a small state situated in the north eastern region of the Indian subcontinent was carried out at Viral Research & Diagnostic Laboratory (VRDL), Department of Microbiology, Regional Institute of Medical Sciences (RIMS), Imphal which is a tertiary care hospital.

Materials & Methods: A total of 914 blood samples from clinically dengue suspected patients were screened for the presence of Dengue infection by adopting ELISA (IgM) technique during the period from 01/06/2022 to 02/12/2022. Further, anti-Dengue IgM antibody positive samples having high Optical Density (OD) value(s) were selected and subjected to RT PCR to determine the serotype(s) of Dengue virus.

Results: Of the 914 blood samples examined for the presence of Dengue infection, 111 (12.14%) were found positive for anti-Dengue IgM antibody indicating acute infection of Dengue virus. Of the positive patients, there were 56 (50.45%) males and 55 (49.54%) females. The sex difference was found to be statistically insignificant (।z।CV = 0.016; z ˂ 1.96; P ˂ 0.05). Predominant clinical features observed among the Dengue confirmed patients included – fever (74%), headache (19%), arthralgia / joint pain (9%), myalgia (6%) and vomiting (6%) respectively. Haemorrhagic manifestation was observed in only one (01) patient. Only three (03) Dengue positive patients had the history of travelling outside the state.

The study revealed that while circulation of three (03) Dengue virus serotypes, namely DENV – 1, DENV – 2 & DENV – 3 were observed in the Tengnoupal district, the circulation of two Dengue virus serotypes i.e., DENV – 1 & DENV – 2 were evident in Imphal West & Bishnupur districts respectively. The present study also reveals the occurrence of Dengue virus serotype – 1 (DENV - 1) in Churachandpur district.

Conclusion: The present study reveals the circulation / distribution of three Dengue virus serotypes namely, DENV – 1, DENV – 2 and DENV – 3 among the studied samples. Findings from this pilot study stresses the need for continuing more in-depth studies among the population of the state towards identifying serotypes endemic/prevalent in the different districts of the state.

Keywords: Dengue virus, Dengue IgM ELISA, Dengue serotype (RT PCR)

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References

1. WHO. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control, Geneva, Switzerland, WHO-TDR publication, 2009; http://www.who.libdoc.who.int-publications 2009-9789241547871_eng pdf

2. WHO. Dengue and Dengue haemorrhagic fever. 2006; Fact sheet No. 117 http://www.who.int/mediacentre/factsheets/fs117/en/print.html

3. WTO. Tourism highlights. 2007; http://www.world-tourism.org/facts/menu.html

4. CDC. Dengue: Epidemiology. 2016; http://www.cdc.gov/dengue/epidemiology

5. WHO. Dengue and Severe dengue. 2023; https://www.who.int/publications/i/items/9789241547871

6. Halstead, S.B. Global perspective on Dengue research. Dengue Bul 2000; 24:77-82

7. Sankari, T., Hoti, S.L., Singh, Th.B. and Shanmugavel, J. Outbreak of dengue virus serotype – 2 (DENV - 2) of Cambodian origin in Manipur, India – Association with meteorological factors. Ind J Med Resh 2012; 136:649-655

8. Khan, S.A., Dutta, P., Borah, J., Chowdhury, P., Doloi, P.K. and Mahanta, J. Dengue outbreak in an Indo-Myanmar border area: epidemiological aspects and risk factors. Trop Biomed 2013; 30(3):451-458

9. WHO. Dengue haemorrhagic fever: Diagnosis, Treatment, Prevention and Control. 1997; 2nd Edition, Geneva

10. Anon. Clinical case definition of Dengue/DHF/DSS (Appendix 4.2); In Information booklet for the Network of Virus Research & Diagnostic Laboratories (VRDLs), Resource Centre, VRDLs, National Institute of Virology, Pune (India). 2019; P-33

11. Bharaj, P., Chachar, H.S., Pandey, A., Diddi, K., Dar, L., Guleria, R., Kabra, S.K. and Broor, S. Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India. Virol J 2008; 5:1 DOI: 10.1186/1743-422X-5-1

12. Sarkar, A., Taraphdar, D. And Chatterjee, S. Molecular typing of Dengue virus circulating in Kolkata, India in 2010. J Trop Med 2012; DOI: 10.1155/2012/960329

13. Chakravarti, A., Matlani, M., Kashyap, B. and Kumar, A. Awareness of changing trends
in epidemiology of dengue fever is essential for epidemiological surveillance. Ind J Med Microbiol 2012; 30 (2):222-226

14. Anoop, M., Issac, A., Mathew, T., Philip, S., Kareem, N.A., Unnikrishnan, R. and Sreekumar, E. Genetic characterization of dengue virus serotypes causing concurrent infection in an outbreak in Ernakulam, Kerala, South India. Ind J Expt Biol 2010; 48:849-857

15. Kurukumbi, M., Wali, J.P., Broor, S., Aggarwal, P., Seth, P., Handa, R., Dhar, L. and Vajapayee, M. Seroepidemiology and active surveillance of dengue fever/dengue haemorrhagic fever in Delhi. Ind J Med Sc 2001; 55(3):149-156

16. Scott, T.W., Naksathit, A., Day, J.F., Kittayapong, P. and Edman, J.D. A fitness advantages for Aedes aegypti and the viruses it transmits when females feed only on human blood. Am J Trop Med Hyg 1997; 57 (2):235-239

17. Lorono-Pino, M.A., Cropp, C.B., Farfan, J.A., Vorndam, A.V., Rodriguez-Angulo, E.M., Rosado-Paredes, E.P., Flores-Flores, L.F., Beaty, B.J. and Gubler, D.J. Common occurrence of concurrent infections by multiple dengue virus serotypes. Am J Trop Med Hyg 1999; 61(5):725-730

18. Ismail, N.A., Kampon, N., Mahdy, Z.A., Jamil, M.A. and Razi, Z.R. Dengue in pregnancy. Southeast Asian J Trop Med Publ Hlth 2006; 37 (4):681-683

19. Waduge, R., Malavige, G.N., Pradeepan, M., Wijeyaratne, C.N., Fernando, S. and Seneviratne, S.L. Dengue infections during pregnancy: A case series from Sri Lanka and review of the literature. J Clin Virol 2006; 37 (1):27-33

20. Adam, L., Jumaa, A.M., Elbashir, H.M. and Karsany, M.S. Maternal and perinatal outcomes of dengue in PortSudan, Eastern Sudan. Virol J 2010; 7:153

21. Pouliot, S.H., Xiong, X., Harville, E., Paz-Soldan, V., Tomashek, K.M., Breart, G. and Buekens, P. Maternal dengue and pregnancy outcomes: A systematic review. Obst Gynaecol Surv 2010; 65 (2):107-118
How to Cite
1.
Laitonjam Anand Singh, Thingujam Suson, Chongtham Geeta Chanu, Khuraijam Ranjana Devi, Heigrujam Rebachandra Singh. Dengue Serotypes In Manipur – Findings From A Retrospective Analytical Study In A Tertiary Care Hospital. Int J Med Res Rev [Internet]. 2024Jan.2 [cited 2024Nov.22];11(6):136-45. Available from: https://ijmrr.medresearch.in/index.php/ijmrr/article/view/1452
Issue
Vol 11 No 6 (2023): Nov-Dec
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Original Article

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